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Item Burnout syndrome prevalence in veterinarians working in Chile(2019) Weinborn, Romy M.; Bruna, Braulio J.; Calventus, Joan; Sepúlveda, Gerardo A.Burnout Syndrome is a psycho-emotional syndrome that affects workers in any activity or profession. In recent years, veterinarians have been described as one of the most affected professionals, which has motivated the development of this research. The primary objective of this work was to determine the prevalence of Burnout Syndrome in veterinarians working in Chile. We evaluated the possible correlation of Burnout Syndrome with socio-demographic variables. The Socio-Demographic Characterization Survey and the Maslach Burnout Syndrome Inventory-General Survey (MBI) were applied to 521 participants, who were contacted through the Veterinary Medical Association of Chile (COLMEVET) and social networks. Fisher and Chi-square statistical tests and correspondence analysis were used to determine the association among variables. The prevalence of Burnout Syndrome in Chilean veterinarians was 24% (124/521). There was statistical significance between Burnout Syndrome and the variables ‘years in employment’ and ‘monthly salary’. There is a high prevalence of Burnout Syndrome in veterinarians working in Chile that was related to the variables ‘years in employment’ and ‘monthly salary’. Prevention using psychological therapy could reduce the incidence of symptoms linked to adaptive difficulties, cognitive discrepancies, psychological discomfort, and emotional regulation, promoting health improvement and resistance to Burnout Syndrome in Chilean veterinarians. This research corresponds to the first exploratory study related to the subject in Chile.Item Humans as blood-feeding sources in sylvatic triatomines of Chile unveiled by next-generation sequencing(2023-07-06) San Juan, Esteban; Araya‑Donoso, Raúl; Sierra‑Rosales, Catalina; Correa, Juana P; Quiroga, Nicol; Campos‑Soto, Ricardo; Solari, Aldo; Llewellyn, Martin; Bacigalupo, Antonella; Botto‑Mahan, CarezzaBackground Triatomines are blood-sucking insects capable of transmitting Trypanosoma cruzi, the parasite that causes Chagas disease in humans. Vectorial transmission entails an infected triatomine feeding on a vertebrate host, release of triatomine infective dejections, and host infection by the entry of parasites through mucous membranes, skin abrasions, or the biting site; therefore, transmission to humans is related to the triatomine–human contact. In this cross-sectional study, we evaluated whether humans were detected in the diet of three sylvatic triatomine species (Mepraia parapatrica, Mepraia spinolai, and Triatoma infestans) present in the semiarid–Mediterranean ecosystem of Chile. Methods We used triatomines collected from 32 sites across 1100 km, with an overall T. cruzi infection frequency of 47.1% (N=4287 total specimens) by conventional PCR or qPCR. First, we amplifed the vertebrate cytochrome b gene (cytb) from all DNA samples obtained from triatomine intestinal contents. Then, we sequenced cytb-positive PCR products in pools of 10–20 triatomines each, grouped by site. The fltered sequences were grouped into amplicon sequence variants (ASVs) with a minimum abundance of 100 reads. ASVs were identifed by selecting the best BLASTn match against the NCBI nucleotide database. Results Overall, 16 mammal (including human), 14 bird, and seven reptile species were identifed in the diet of syl‑ vatic triatomines. Humans were part of the diet of all analyzed triatomine species, and it was detected in 19 sites representing 12.19% of the sequences. Conclusions Sylvatic triatomine species from Chile feed on a variety of vertebrate species; many of them are detected here for the frst time in their diet. Our results highlight that the sylvatic triatomine–human contact is noteworthy. Education must be enforced for local inhabitants, workers, and tourists arriving in endemic areas to avoid or minimize the risk of exposure to Chagas disease vectors.Item Bartonella Infection in Stray Dogs from Central and Southern Chile (Linares and Puerto Montt)(Vector-Borne and Zoonotic Diseases, 2020) Weinborn-Astudillo, Romy M.; Pau, Natalia; Tobar, Bret Z.; Jaffe, David A.; Boulouis, Henri-Jean; Sepúlveda, Paulina; Müller, Ananda; Chomel, Bruno B.Bartonellae are emerging zoonotic vector-borne pathogens causing a broad spectrum of clinical symptoms in humans and animals, including life-threatening endocarditis. Dogs are infected with a wide range of Bartonella species and infection has been reported in free-roaming dogs from various South American countries. We report a high Bartonella seroprevalence in 82 Chilean stray dogs. More than half of the dogs from Linares (72.7%, n = 66) and Puerto Montt (56.2%, n = 16) were seropositive for Bartonella henselae, Bartonella vinsonii ssp. berkhoffii, or Bartonella clarridgeiae with antibody titers ranging from 1:64 to 1:512. Three dogs (3.6%) were PCR positive for Bartonella sp. Partial sequencing of the gltA gene indicated that two dogs were infected with B. henselae, and one with a strain close to Bartonella vinsonii ssp. vinsonii. Exposure to Bartonella species was common in stray Chilean dogs, as for other South American countries, likely associated with heavy ectoparasite infestation.Item Brucelosis en personas con riesgo ocupacional en clínicas veterinarias de dos ciudades del centro sur de Chile(2020-11-25) Weinborn A., Romy; Zanelli G., Macarena; Troncoso T., Ignacio; Opazo V., Álvaro; Valenzuela A., Karina; Cárdenas Z., Sebastián; García, Rodrigo; Vásquez A., SamuelEl objetivo del estudio fue determinar la seroprevalencia de Brucella spp en personas con riesgo ocupacional en clínicas veterinarias de dos ciudades del centro sur de Chile (Talca y Puerto Montt). Se analizaron 98 sueros humanos mediante la técnica de aglutinación Wright-Huddleson (sensibilidad 54.9%, especificidad 100%) y se utilizó el test exacto de Fischer o X2 para evaluar diferencias significativas entre grupos (p<0.05). Se encontró 3% de seropositividad a Brucella spp (1.8% [1/53] para Talca y 4.4% [2/45] para Puerto Montt). Según las funciones realizadas dentro de las clínicas veterinarias, la seropositividad fue de 1.4% (1/71) para alumnos de medicina veterinaria y 10% (2/19) en médicos veterinarios, sin diferencias significativas entre grupos.