The use of filamentous hemagglutinin adhesin to detect immune responses to Campylobacter hepaticus infections in layer hens

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dc.contributor.authorMuralidharan, Chithralekha
dc.contributor.authorQuinteros, José A.
dc.contributor.authorAnwar, Arif
dc.contributor.authorWilson, Timothy B.
dc.contributor.authorScott, Peter C.
dc.contributor.authorMoore, Robert J.
dc.contributor.authorHao Van, Thi Thu
dc.date.accessioned2023-04-11T19:20:12Z
dc.date.available2023-04-11T19:20:12Z
dc.date.issued2022-12-21
dc.description.abstractCampylobacter hepaticus is the aetiological agent of Spotty Liver Disease (SLD). SLD can cause significant production loss and mortalities among layer hens at and around peak of lay. We previously developed an enzyme linked immunosorbent assay (ELISA), SLD-ELISA1, to detect C. hepaticus specific antibodies from bird sera using C. hepaticus total proteins and sera pre-absorbed with Campylobacter jejuni proteins. The high specificity achieved with SLD-ELISA1 indicated the presence of C. hepaticus specific antibodies in sera of infected birds. However, some of the reagents used in SLD-ELISA1 are time consuming to prepare and difficult to quality control. This understanding led to the search for C. hepaticus specific immunogenic proteins that could be used in recombinant forms as antibody capture antigens in immunoassay design. In this study, an immunoproteomic approach that combined bioinformatics analysis, western blotting, and LC MS/MS protein profiling was used, and a fragment of filamentous hemagglutinin adhesin (FHA), FHA1,628−1,899 with C. hepaticus specific antigenicity was identified. Recombinant FHA1,628−1,899 was used as antigen coating on ELISA plates to capture FHA1,628−1,899 specific antibodies in sera of infected birds. SLD-ELISA2, based on the purified recombinant FHA fragment, is more user-friendly and standardizable than SLD-ELISA1 for screening antibody responses to C. hepaticus exposure in hens. This study is the first report of the use of FHA from a Campylobacter species in immunoassays, and it also opens future research directions to investigate the role of FHA in C. hepaticus pathogenesis and its effectiveness as a vaccine candidate.es_ES
dc.identifier.issn2297-1769
dc.identifier.other10.3389/fvets.2022.1082358
dc.identifier.urihttps://hdl.handle.net/20.500.12536/1930
dc.language.isoenes_ES
dc.sourceFrontiers in Veterinary Sciencees_ES
dc.subjectCampylobacter hepaticuses_ES
dc.subjectSpotty Liver Diseasees_ES
dc.subjectELISAes_ES
dc.subjectFilamentous hemagglutinin adhesiones_ES
dc.subjectSeraes_ES
dc.subjectImmunoassayes_ES
dc.titleThe use of filamentous hemagglutinin adhesin to detect immune responses to Campylobacter hepaticus infections in layer henses_ES
dc.typeArtículo de revistaes_ES
uvm.carreraMedicina Veterinariaes_ES
uvm.escuelaEscuela de Ciencias Agrícolas y Veterinariases_ES
uvm.indexScopuses_ES
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